FIGURE 4.

The self-assembly state of Drp1 differentially regulates its mitochondrial recruitment through MIEFs or Mff (A) Confocal images of mitochondrial morphology and endogenous Drp1 distribution in wild-type (WT) or Drp1^−/− 293T cells stained with MitoTracker (red) and anti-Drp1 (green) antibody. (B–F) Confocal images of mitochondrial morphology and exogenous Drp1 distribution in Drp1^−/− 293T cells co-transfected with Drp1-WT or an indicated Drp1 mutant and empty vector, MIEF1-V5, MIEF2-V5 or Myc-Mff. At 16 h post-transfection, cells were stained with MitoTracker (red) before fixation, followed by immunostaining with anti-Drp1 (green) and anti-V5 or anti-Myc (blue) antibodies. (Yellow color indicates co-localization of Drp1 with mitochondria. White color indicates co-localization of Drp1 and the respective receptor protein on mitochondria.)