Figure 4.

HIVEP1-deficient murine macrophages show increased proinflammatory cytokine production upon stimulation with LPS. Bone marrow derived macrophages from hivep1^-/- mice and wild type littermates were stimulated with LPS (100 ng/mL). Hivep1^-/- macrophages showed increased expression of TNF-α, IL-1β, IL-6, CXCL1 mRNA upon LPS stimulation (A–D), as well as enhanced TNF-α, IL-6 and CXCL1 protein release (E–G). Data shows means ± SEM and were pooled from three independent data set [n = 4 (A–D) or n = 12 (E–G) for each condition within each experiment)]. *P < 0.05, **P < 0.01, ***P < 0.001.