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. 2021 Oct 29;148(21):dev199644. doi: 10.1242/dev.199644

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — FAM71F1 is localized to the Golgi apparatus and interacts with RAB2A and RAB2B. (A) Domain structure of mouse FAM71F1. FAM71F1 contains a RAB2B-binding domain (green). (B) Immunofluorescence of FAM71F1 and Golgi apparatus using COS-7 cells. HA-tagged mouse Fam71f1 was transiently expressed. FAM71F1-HA colocalized with GM130-positive Golgi apparatus. Nuclei are stained blue (Hoechst), Golgi apparatus green (GM130) and FAM71F1-HA magenta (HA). (C) The FAM71F1 proteome identified by immunoprecipitation-mass spectrometry analysis. Testis lysates from wild-type (+/+) or Fam71f1-PA Tg (−4/−4; Tg) mice were immunoprecipitated using anti-PA antibodies and associated proteins were identified by mass spectrometry. Proteins either identified only in PA-tagged Fam71f1 Tg mice (1-10) or proteins with a high ratio in the Tg immunoprecipitation are listed (11-13). The remaining mass spectrometry results are shown in Table S1. Ratio=(−4/−4; Tg)/(+/+) (quantitative value). (D) Amino acid sequence of RAB2A and RAB2B. Peptide sequences of RAB2A and RAB2B that were identified by mass spectrometry are indicated by colored boxes; common sequences in RAB2A and RAB2B are in orange; specific sequences in RAB2A in magenta; and a specific sequence in RAB2B in green. Light-blue shading indicates amino acid residues common to both RAB2A and RAB2B. Scale bar: 20 μm in B.