TABLE 3.

Defect in hematopoietic colony formation by yolk sac cells from Fli1 mutant mice

Embryo no.	Genotype	No. of cells/fetusa
		CFU-E	BFU-E	CFU-mix	CFU-GM
Expt 1
1	+/+	375 ± 45	150 ± 30	135 ± 75	68 ± 45
2	+/+	615 ± 105	165 ± 15	480 ± 75	75 ± 45
3	+/+	405 ± 75	300 ± 135	128 ± 30	45 ± 15
4	+/+	1,035 ± 90	360 ± 180	405 ± 75	105 ± 6
5	+/−	1,320 ± 270	375 ± 105	225 ± 60	30 ± 30
6	+/−	1,035 ± 150	405 ± 105	113 ± 30	45 ± 6
7	−/−	285 ± 30	83 ± 30	8 ± 8	8 ± 6
8	−/−	540 ± 135	150 ± 60	8 ± 8	15 ± 15
Expt 2
1	+/+	70 ± 30	40 ± 10	410 ± 60	150 ± 60
2	+/+	170 ± 40	350 ± 80	440 ± 20	90 ± 40
3	+/+	180 ± 20	200 ± 60	390 ± 80	150 ± 10
4	+/−	370 ± 100	300 ± 30	170 ± 10	100 ± 30
5	+/−	400 ± 120	320 ± 60	170 ± 40	210 ± 30
6	+/−	350 ± 100	170 ± 10	200 ± 10	180 ± 0
7	+/−	770 ± 90	320 ± 110	240 ± 20	230 ± 50
8	+/−	310 ± 50	130 ± 20	90 ± 40	30 ± 10
9	−/−	220 ± 30	100 ± 60	60 ± 20	20 ± 10
10	−/−	150 ± 40	80 ± 20	50 ± 20	20 ± 20
11	−/−	260 ± 50	110 ± 30	50 ± 20	40 ± 20
12	−/−	190 ± 70	100 ± 20	40 ± 30	40 ± 20
13	−/−	210 ± 20	100 ± 30	100 ± 10	20 ± 10

^aMean ± standard deviation of quadruplicate cultures containing 1/10 yolk sac (experiment 1) or 1/15 yolk sac (experiment 2) and IL-3, IL-6, SF, and Epo. CFU-E cells were scored on day 4, and the other colony types were scored on day 7.