1M PB buffer (pH 7.4)
| Reagent | Final concentration | Amount |
|---|---|---|
| 2M NaH2PO4. 2H2O (monobasic sodium phosphate) | 1M | 47.5 mL |
| 2M Na2HPO4 (dibasic sodium phosphate) | 1M | 202.5 mL |
| ddH2O | n/a | 250 mL |
| Total | 1M | 500 mL |
Keep at 22°C–25°C.
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