Embedding medium Mowiol-DABCO

Reagent	Final concentration	Amount
Glycerol	n/a	6 g
Mowiol	n/a	4 g
ddH2O	n/a	6 mL
0.2M Tris-HCl (pH 8.5)	0.1	12 mL
DABCO	n/a	25 mg
Total	n/a	25 mL

Details for Mowiol-DABCO preparation: (1) Use a balance to weight 6.0 g Glycerol and 2.4 g Mowiol. Given the high viscosity of Glycerol, it is more accurate to measure its weight than its volume. (2) Mix both reagents and dissolve for 1 h on a stirring plate. (3) Add 6 mL distilled water and mix for another hour. (4) Add 12 mL 0.2M Tris-HCl (pH 8.5) and incubate for 2 h at 50°C on a stirring heating plate. (5) It is likely that not all Mowiol is going to dissolve. Thus, you can add an additional centrifugation step to remove undissolved Mowiol from the preparation by centrifuging for 15 min at 5,000 g and keeping the supernatant. (6) Add 25 mg DABCO and dissolve so that you have a clear solution. (7) Aliquot in 1.5 mL tubes and store at −20°C until further usage.