Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Anesthesia working solution (2.5%)
| Reagent | Final concentration | Amount |
|---|---|---|
| 100% anesthesia stock solution | 2N | 0.875 mL |
| 1× PBS | 1× | 34.125 mL |
| Total | 2.5% | 35 mL |
Details for anesthesia preparation: (1) Vortex until the solution appears homogeneous. (2) Filter the solution into a fresh tube using a 60 mL syringe and a 0.2micron filter. (3) Store at 4°C for up to one month.
