Figure 1.

α-Synuclein sequence and principles of LD measurements. (A) α-Synuclein primary structure has three main regions: the N-terminal domain, highlighted in light gray, is rich in positively charged residues and determines the protein binding to negatively charged membranes; the nonamyloid-β component (NAC) domain, underline, forms the core of the amyloid fibrils, and the C-terminal domain, dotted underlined sequence, is rich in negatively charged residues. (B) Orientation of polarization transition moments in α-helix structure. (C) Couette flow cell, the alignment technique used in this study. Adapted with permission from ref (30). Copyright 2014 National Academy of Sciences.