Table 1. Order Parameter S of DOPS, POPS, and DOPG Vesicles before and Immediately after Addition of α-Synuclein (t0 Corresponds to the First Measurement, When the Flow Starts) and Angles of the Protein π–π* Transition at 208 nm (Helix Long Axis) Relative to the Membrane Normal for the Conditions That Could Be Fitted by Eq 2 (Ellipsoid Model); Angles Obtained Immediately after Adding the Protein (t0) and after 30 min under Flow (t30 min) Are Shown.
DOPS |
POPS |
DOPG |
||||
---|---|---|---|---|---|---|
S factor | L:P 100 | L:P 200 | L:P 100 | L:P 200 | L:P 100 | L:P 200 |
vesicles only, t0 | 0.081 | 0.074 | 0.069 | 0.074 | 0.019 | 0.014 |
α-synuclein addition, t0 | 0.072 | 0.069 | 0.055 | 0.063 | 0.030 | 0.026 |
angle γ (deg) at 208 nm | ||||||
α-synuclein addition, t0 | 90 | 78 | ||||
α-synuclein addition, t30 min | 84 | 65 | 90° | 66 | 80 | 80 |