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. 2021 Jun 2;32(11):2295–2306. doi: 10.1021/acs.bioconjchem.1c00179

Figure 1.

Figure 1

Structure of drugs and conjugates and the research strategy implemented in this study. (a) Chemical structures of heroin, 6-AM, morphine, and fentanyl. (b) Structures of TT-6-AmHap and TT-para-AmFenHap conjugates. The 6-AmHap and para-AmFenHap haptens and the NHS-(PEG)2-maleimide cross-linker [SM(PEG)2] linker are depicted in red, blue, and black, respectively. (c) Research strategy: TT-6-AmHap (10 μg) and TT-para-AmFenHap (10 μg) conjugates were mixed with ALF43 and Alhydrogel (ALF43A) adjuvant, and injected i.m. to female Balb/c mice. Adjuvant doses were the same in monovalent and bivalent formulations. The ability of the serum IgG to sequester the opioids in vitro and block their anti-nociceptive effects in vivo was tested.