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. 2021 Jun 2;32(11):2295–2306. doi: 10.1021/acs.bioconjchem.1c00179

Figure 2.

Figure 2

Immune response to haptens. Mice (n = 10 per group) were immunized at weeks 0, 3, 6, and 14 with the monovalent or bivalent vaccine formulation. Monovalent formulation used 10 μg of antigen. Bivalent vaccine was composed of 10 μg TT-para-AmFenHap and 10 μg TT-6-AmHap. Immunogenicity was evaluated on sera collected on week 16 using ELISA with the indicated coating antigen: (a) animal study timeline; hapten-specific IgG end point titers to (b) BSA-6-AmHap and (c) BSA-para-AmFenHap. Data shown are mean ± SEM. Statistical differences were tested using Mann–Whitney nonparametric t-test (**** p < 0.0001; **, p < 0.005; *, p < 0.05).