Figure 6.

Cross-reactivity of bivalent vaccine to therapeutics in vivo. Mice (n = 10 per group) were challenged s.c. with increasing doses of either buprenorphine or methadone. The anti-nociceptive effects were assessed by hotplate assay 15 min after drug dosing and reported as %MPE: (a) buprenorphine, (b) methadone, and (c) experimental flow to assess the cross-reactivity of naloxone in vivo. Mice (n = 10 per group) were challenged s.c. with 0.50 mg/kg heroin with 0.05 mg/kg fentanyl; 15 min afterwards, mice received 0.1 mg/kg naloxone, s.c. The thermal nociception was assessed 5 and 20 min post-naloxone. (d) Anti-nociceptive effects of heroin + 9% (w/w) fentanyl in the presence or absence of naloxone. Data shown are mean ± SEM. Statistical significance (naloxone vs saline group) was determined using a two-tailed, unpaired t-test. ***, p < 0.0005, ****, p < 0.0001. Nlx, naloxone, Sal, saline.