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. 2021 Nov 2;143(45):18877–18887. doi: 10.1021/jacs.1c02156

Figure 2.

Figure 2

ArchT can be activated to repeatedly and robustly raise pHi in single cells. (A) Individual RPE cells expressing ArchT and pH biosensor (pHluorin) were stimulated using the standard protocol (see Methods) followed by 2 min recovery. This pattern is repeated 9 times. (B) Single-cell pHluorin intensity traces of single ArchT (blue) and Control (black) cells treated as described in (A). (C) Average (mean) cell data collected as in B. (n = 9–10 per condition, 3–4 biological replicates). (D) Change in pH intensity quantified for cells in (C), mean ± SEM. For part D, significance compared to Stim 1 determined using the two-way ANOVA, with Holm-Sidak multiple comparison correction, ** p < 0.01.