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. Author manuscript; available in PMC: 2021 Nov 19.
Published in final edited form as: Biochemistry. 2021 Jan 5;60(2):152–159. doi: 10.1021/acs.biochem.0c00863

Figure 4.

Figure 4.

Correlations of solution- and dry-state protection. (A) % protected after freeze-drying in buffer vs average opening free energy (ΔGop°′) in buffer for the 34 GB1 residues for which solution HDX rates could be measured. (B) Average Δ% protected due to drying with 20 g/L urea or trehalose vs the change in opening free energy (ΔΔGop°′) due to the presence of the same cosolute at 100 g/L (ΔΔGop°′ = ΔGop,cosolute°′ − ΔGop,buffer°′). % protected and ΔGop°′ values are reported with their uncertainties in Tables S4 and S5, respectively.