FIG. 7.

Mutations in predicted loop regions and at the predicted borders of β-strand 3 have moderate to no effect on the interaction with RAG1 and hence on the capacity to form coding and signal joints in vivo. Eight drastic mutations, E280A, N295A, D306N, N335L-Q337L, E341Q, S356L, E357L-D358A, and S340A, were introduced into the variable predicted loop regions of RAG2, and these substitutions were tested for the ability to mediate signal joint and coding-joint formation in vivo (A) and for the capacity to precipitate RAG1 (B). WT, wild type.