Extended Data Fig. 10 ∣. Skin-resident immune cells actively coordinate their distribution with epithelial basal cells.

During homeostasis, LCs and DETCs actively maintain a non-random distribution. Although these immune cells continuously move within epithelial basal cell neighbors, they sustain their regular tiling pattern. Moreover, epithelial basal cells determine the density of LCs and DETCs in the skin epidermis. However, LCs and DETCs do not impact the density of epithelial basal cells nor the architecture of the epidermis signifying a unidirectional regulation of density. Finally, immune cell dendrites enable maximal coverage of the basal layer and do not come into contact with neighbouring immune cells in homeostasis and their dendritic behaviors, mediated by Rac1, coordinate their patterned organization.