Fig. 1.

Loss of CD44 inhibited HCC progression. A Kaplan–Meier curves show the overall survival of patients with high or low expression of CD44 in HCC. Statistical significance was determined by a log-rank test. B Cell viability was measured in SMMC-7721 and MHCC-97 H cells with CD44 knockdown using siRNAs, and the CCK8 assay was performed to analyze cell proliferation. C–F The colony formation assay, wound-healing, migration and invasion assay were performed in SMMC-7721 and MHCC-97 H cells with CD44 depletion. All data are the mean ± SD, n = 3, t-test, *P < 0.05, **P < 0.01, ***P < 0.001