Fig. 2.

CD44 facilitated HCC progression via regulating Hippo-YAP signaling. A Overexpression or knockdown of CD44 in HCC cells, lysates were prepared and blotted with the indicated antibodies. B The cellular localization of YAP protein was determined by immunofluorescence in MHCC-97 H cells transfected with CD44 siRNAs, Scale bar represent 20 μm. C Representative western blots showed that over-expression of YAP in SMMC-7721 cells with CD44 knockdown. D–F Cell viability, wound-healing assay and invasion assay were performed on CD44-depleted SMMC-7721 cells with YAP overexpression, scaled bars represent 400 μm. G Quantification of the invaded cells in (F). All data are the mean ± SD, n = 3, t-test,*P < 0.05, ***P < 0.001