Fig. 8.

Aβ-Th1 and Aβ-Th17 cells affect neurogenesis and synaptic plasticity in APP/PS1 mice. a Neuronal progenitor cell density determined by immunohistochemical staining of doublecortin positive (Dcx+) cells in the dentate gyrus region of the hippocampus. Representative images showing Dcx+ cells. Scale bar = 20 µm. Dcx+ cells were quantified using the optical fractionator module of Stereo Investigator system (MBF Bioscience). n = 6 mice per group were analyzed. b Western blot analyses performed to determine expression of presynaptic (synaptophysin) and postsynaptic (PSD95) neurons using cortical tissue protein. Representative immunoblot and densitometric quantification performed for n = 4 mice per group. Statistical significance between groups determined using one-way ANOVA followed by Newman–Keuls post hoc test. *p < 0.05, ****p < 0.0001