Figure 8:

Printing constructs with heterogeneous cell alignment. Representative schematics and images of 5% GelMA printed constructs with (A) circle-cross design and (B) multi-material print (fibers: grey, no fibers: blue) to illustrate the potential to print structures with variable cell alignment behavior within a single construct (scale bars 1 mm). Confocal images showing fibers (grey) and MFCs (F-actin: red; cell nuclei: blue) for printed constructs (middle) 7 days after culture (30 μm z-stacks, scale bars 0.1 mm) and quantification of fiber and cell alignment along printed filament direction 7 days after culture (n=3 for each group, mean ± s.d., Watson-Wheeler test *p ≤ 0.05, *** p ≤ 0.001)