REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Panx-1	Thermofisher	Cat#487900, RRID: AB_2532253
Iba-1	Abcam	Cat#ab5076, RRID: AB_2224402
EpCAM	Abcam	Cat#ab7504, RRID: AB_305949
Donkey Anti-Rabbit Alexa 488	Thermo Fisher	Cat#A21206, RRID:AB_2535792
Donkey Anti-Goat Alexa 568	Thermo Fisher	Cat#A11057, RRID:AB_2534104
Goat Anti-Mouse Alexa 568	Thermo Fisher	Cat#A11031, RRID: AB_144696
Biological samples
Primary Small Airway Epithelial Cells; Normal, Human	ATCC	Cat#PCS-301-010
Cell lines
Airway Epithelial Cell Basal Medium	ATCC	Cat#PCS-300-030
Chemicals
DMEM	ThermoFisher Scientific	Cat# 11995-065
Bronchial Epithelial Growth Kit	ATCC	Cat#PCS-300-040
Dulbecco’s Phosphate-buffered saline	gibco	Cat#14190-144
Dako Pen	Dako	Cat# S2002
0.05% Trypsin EDTA	ThermoFisher Scientific	Cat# 25300-054
Penicillin/Streptomycin	ThermoFisher Scientific	Cat#15140-122
Fetal bovine serum	ThermoFisher Scientific	Cat# 16000044
Paraformaldehyde	Electron Microscopy Sciences	Cat# 15710-S
Fish gelatin	Sigma-Aldrich	Cat# G7041
BSA (Bovine serum albumin)	Sigma-Aldrich	Cat# 05470
Horse serum	Sigma-Aldrich	Cat# H0146
EDTA	Invitrogen	Cat#15575-020
Xylene	Fisher Chemical	Cat#X3S-4
Ethanol 200proof	Decon Labs, Inc.	Cat#2701
Tris-buffered saline	Fisher-Bioreagents	BP2471-1
Triton x-100	Sigma	X100
Ethidium Bromide Solution	ThermoFisher Scientific	Cat#15585-011
ProLong™ Gold Antifade Mounting with DAPI	ThermoFisher Scientific	Cat# P36931
SigmaFast DAB Tablet	Sigma-Aldrich	Cat#D-4168
Chemicals
RNAscope Target retrieval solution	ACD	Cat# 322000
RNAscope Wash Buffer	ACD	Cat# 310091
RNAscope Multiplex Fluorescent Detection Reagents V2	ACD	Cat# 323110
RNAscope LS Multiplex TSA Buffer	ACD	Cat# 322810
Probenecid	Sigma	Cat# P8761-25G
Panx-1 mimetic blocking peptide	Origene	Sequence: WRQAAFVDSY
Panx-1 scrambled peptide	Origene	Sequence: FADRYWAQVS
SARS-CoV-2 Spike Membrane protein	Origene	Cat# TP701119
hCoV-229E Spike Membrane protein	Origene	Cat# VC100563
Probes
SARS-CoV-2 (Sense) probe	ACD	Cat# 845701
SARS-CoV-2 (S) probe	ACD	Cat# 848561
Software
NIS-Elements-AR	Nikon	https://www.microscope.healthcare.nikon.com
Nano Zoomer Digital Pathology	Hamamatsu	https://www.hamamatsu.com/us/en/product/type/U12388-01/index.html