FIG. 4.
Unlike Bcl-xL, ROS scavengers cannot protect cells from the initial decrease in ΔΨm following TNF-α treatment. TMRE staining followed by FACS analysis was used to monitor the ΔΨm in untreated cells (shaded area), in cells treated with TNF-α for 3 h (thick line) or in cells treated with FCCP for 3 h (dashed line). Only live cells (as judged by forward-scatter and side-scatter criteria) were analyzed. (A) Control cells (2B4/neo). (B) Bcl-xL-expressing cells (2B4/Xl c.10). (C) 2B4/neo cells treated as above in the presence of the ROS scavenger PDTC.