FIG. 6.
Inhibition of respiration induced by TNF-α is reversed by Bcl-xL expression. (A) Control cells (2B4/neo) and Bcl-xL-expressing cells (2B4/Xl c.10) were either left untreated or treated with TNF-α for 2 h. Oxygen consumption was analyzed using an oxymeter (see Materials and Methods), and the decrease in oxygen pressure in the chamber over time is presented for each sample. (B) For each treatment, the average slopes of the graphs obtained in panel A, calculated from three independent experiments, are presented. This value describes the average rate of oxygen consumption by the cells (presented as the decrease in oxygen pressure per second per 106 cells).