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The effect of ΔilvD mutation on growth of bacilli on hemoglobin. (A) Growth curve of WT and ΔilvD B. anthracis in BSM No BCAAs, supplemented with a gradient of Hb(h): 0.1 to 10 μM. (B) WT and ΔilvD B. anthracis in BSM No BCAAs, each supplemented with 10 μM either Hb(h) or Hb(m). (C) Growth curve comparing WT and ΔilvD B. anthracis in LB broth. Error bars on all graphs represent the standard deviations in optical density, at 600 nm, between replicates at each time point.
