FIG 2.

LbcA can form independent complexes with CtpA and its substrates. Each panel shows data from immunoblot analyses of input lysates and immunoprecipitates (Anti-FLAG IP). Strain genotypes are shown above each lane. Polyclonal antisera used for detection are shown at the left. (a) Baits were LbcA-FLAG or the LbcA negative control. (b) Baits were CtpA-S302A–FLAG–His₆ or the CtpA-S302A–His₆ negative control. (c) Baits were PA1198-FLAG or the PA1198 negative control. Immunoblots are single representatives of results from several independent replicate experiments.