KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-AIF1 (IBA1) | Wako | Cat#019-19741; RRID: AB_839504 |
| Rabbit polyclonal anti-ALDH1A1 | Abcam | Cat#ab23375; RRID: AB_2224009 |
| Rabbit polyclonal anti-ALDH1A2 | Sigma-Aldrich | Cat#HPA010022; RRID: AB_1844723 |
| Goat polyclonal anti-AMH (MIS) | Santa Cruz Biotechnology | Cat#sc-6886; RRID: AB_649207 |
| Rabbit polyclonal anti-AR | Santa Cruz Biotechnology | Cat#sc-816; RRID: AB_1563391 |
| Rat monoclonal anti-CD45 (clone 30-F11) | BioLegend | Cat#103101; RRID: AB_312966 |
| Rat monoclonal anti-CDH1 (clone ECCD-2) | Thermo Fisher Scientific | Cat#13-1900; RRID: AB_2533005 |
| Rabbit polyclonal anti-CLDN11 | Thermo Fisher Scientific | Cat#36-4500; RRID: AB_2533259 |
| Rabbit polyclonal anti-cleaved Caspase 3 (Asp175) | Cell Signaling Technology | Cat#9661S; RRID: AB_2341188 |
| Goat polyclonal anti-CTNNB1 | Santa Cruz Biotechnology | Cat#sc-1496; RRID: AB_1563968 |
| Goat polyclonal anti-CYP17A1 | Santa Cruz Biotechnology | Cat#sc-46081; RRID: AB_2088659 |
| Rabbit polyclonal anti-DDX4 (MVH) | Abcam | Cat#ab13840; RRID: AB_443012 |
| Rat monoclonal anti-GATA1 (clone N6) | Santa Cruz Biotechnology | Cat#sc-265; RRID: AB_627663 |
| Goat polyclonal anti-GATA4 | Santa Cruz Biotechnology | Cat#sc-1237; RRID: AB_2108747 |
| Rabbit polyclonal anti-GDNF | Santa Cruz Biotechnology | Cat#sc-328; RRID: AB_2247684 |
| Goat polyclonal anti-GFRA1 | Neuromics | Cat#GT15004; RRID: AB_2307379 |
| Rabbit polyclonal anti-Histone H2A.X, phospho (Ser139) | Millipore | Cat#07-164; RRID: AB_310406 |
| Guinea pig polyclonal anti-H1F6 (H1T) | Mary Ann Handel (Inselman et al., 2003) | N/A |
| Rat monoclonal anti-ITGA6 (CD49f) (clone GoH3) | BioLegend | Cat#313602; RRID: AB_345296 |
| Goat polyclonal anti-ITGB1 | Novus/R&D Systems | Cat#AF2405-SP; RRID: AB_416591 |
| Goat polyclonal anti-KIT | R&D Systems | Cat#AF1356; RRID: AB_354750 |
| Rabbit monoclonal anti-MKI67 (Ki67) (clone SP6) | GeneTex | Cat#GTX16667; RRID: AB_422351 |
| Rabbit polyclonal anti-PARD3 (Par3) | Novus | Cat#NBP1-88861; RRID: AB_11056253 |
| Goat polyclonal anti-PECAM1 | R&D Systems | Cat#AF3628; RRID:AB_2161028 |
| Rat monoclonal anti-PECAM1 (clone MEC13.3) | BD Biosciences | Cat#553370; RRID: AB_394816 |
| Rabbit monoclonal anti-phospho-Scribble (Ser1220) (clone D8A2) | Cell Signaling Technology | Cat#12316; RRID: AB_2797883 |
| Rabbit polyclonal anti-SOX9 | Millipore | Cat#AB5535; RRID: AB_2239761 |
| Rabbit polyclonal anti-STRA8 | Abcam | Cat#ab49602; RRID: AB_945678 |
| Rat monoclonal anti-TRA98 (clone TRA98) | Abcam | Cat#ab82527; RRID: AB_1659152 |
| Mouse monoclonal anti-TUBB3 (clone TUJ1) | BioLegend | Cat#801201; RRID: AB_2313773 |
| Chicken polyclonal anti-VIM | BioLegend | Cat#919101; RRID: AB_2565208 |
| Rat monoclonal anti-VIM (clone W16220A) | BioLegend | Cat#699301; RRID: AB_2716136 |
| Mouse monoclonal anti-ZBTB16 (PLZF) (clone 2A9) | Millipore | Cat#OP128-100UG; RRID: AB_213280 |
| Alexa Fluor 647-AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | Cat#712-605-153; RRID:AB_2340694 |
| Alexa Fluor 647 Donkey anti-Goat IgG (H+L) | Thermo Fisher Scientific | Cat#A-21447; RRID:AB_2535864 |
| Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat#A-31573; RRID:AB_2536183 |
| Alexa Fluor 647 Goat anti-Mouse IgG2a | Thermo Fisher Scientific | Cat#A-21241; RRID:AB_2535810 |
| Cy3 AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson Immunoresearch | Cat#712-165-153; RRID:AB_2340667 |
| Alexa Fluor 555 Donkey anti-Goat IgG (H+L) | Thermo Fisher Scientific | Cat#A-21432; RRID:AB_2535853 |
| Alexa Fluor 555 Donkey anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat#A-31572; RRID:AB_162543 |
| Alexa Fluor 555 Goat anti-Mouse IgG2a | Thermo Fisher Scientific | Cat#A-21137; RRID:AB_2535776 |
| Alexa Fluor 488 Donkey anti-Goat IgG (H+L) | Thermo Fisher Scientific | Cat#A-11055; RRID:AB_2534102 |
| Alexa Fluor 488 Donkey anti-Rat IgG (H+L) | Thermo Fisher Scientific | Cat#A-21208; RRID:AB_141709 |
| Alexa Fluor Plus 488 Donkey anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat#A32790; RRID:AB_2762833 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) | Jackson Immunoresearch | Cat#703-545-155; RRID:AB_2340375 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor 647 Phalloidin | Thermo Fisher Scientific | Cat#A22287; RRID: AB_2620155 |
| Rhodamine Phalloidin | Thermo Fisher Scientific | Cat#R415; RRID: AB_2572408 |
| Hoechst 33342, Trihydrochloride, Trihydrate | Thermo Fisher Scientific | Cat#H1399 |
| Streptavidin, Alexa Fluor 555 Conjugate | Thermo Fisher Scientific | Cat#S21381; RRID: AB_2307336 |
| EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format | Thermo Fisher Scientific | Cat#A39257 |
| Deposited data | ||
| Fetal gonad (E11.5-E13.5) cell-type-specific microarray dataset | (Jameson et al., 2012) | GEO: GSE27715; GDS3995 |
| Fetal testis (E10.5-E16.5) single-cell (NR5A1-eGFP+ cells) RNA-Seq dataset | (Stévant et al., 2018) | GEO: GSE97519 |
| Adult testis single-cell RNA-Seq dataset | (Green et al., 2018) | GEO: GSE112393 |
| Experimental models: organisms/strains | ||
| Mouse: Dhh-Cre: Tg(Dhh-cre)1Mejr | Dies Meijer; (Jaegle et al., 2003; Lindeboom et al., 2003) | RRID: IMSR_JAX:012929 |
| Mouse: Cdc42 fl/fl: Cdc42tm1Yizh/J | Yi Zheng; (Chen et al., 2006) | RRID: IMSR_JAX:027576 |
| Mouse: CD-1 IGS Mouse: Crl:CD1(ICR) | Charles River | Cat#CRL:022; RRID: IMSR_CRL:022 |
| Mouse: C57BL/6J Mouse: JAX:C57BL/6J | The Jackson Laboratory | Cat#JAX:000664; RRID: IMSR_JAX:000664 |
| Mouse: KitW/KitW-v: WBB6F1/J-KitW/KitW-v/J | The Jackson Laboratory | Cat#JAX:100410; RRID: IMSR_JAX:100410 |
| Mouse: Dnd1Ter: Dnd1Ter | Blanche Capel; (Stevens, 1973; Youngren et al., 2005) | MGI:2158668; RRID: MGI:3690452 |
| Oligonucleotides | ||
| Primers for quantitative real-time PCR, see Table S1 | This study | N/A |
| Software and algorithms | ||
| ImageJ | (Schneider et al., 2012) | https://imagej.nih.gov/ij/ |
| The ReproGenomics Viewer | (Darde et al., 2019; Darde et al., 2015) | https://rgv.genouest.org/ |