Fig. 1.

Correlations of HHQ, NHQ and HQNO detected in sputum, plasma and urine with live P. aeruginosa load by qPCR in sputum during clinical stability. Median (interquartile range) for sputum live P. aeruginosa load by qPCR was 3.3×10⁶ c.f.u. g⁻¹ (1.4×10⁵–1.4x10⁷ c.f.u. g⁻¹) of sputum. HHQ: 2-heptyl-4-hydroxyquinoline; NHQ: 2-nonyl-4-hydroxyquinoline. HQNO: 2-heptyl-4-hydroxyquinoline-N-oxide; Sputum, n=75; plasma, n=74; urine, n=75, where n is the number of participants with data available for statistical analysis. Lowest limit of quantification (LLOQ) for plasma and urine AQs: HHQ (10, 20 pmol l⁻¹), NHQ (10, 10 pmol l⁻¹) and HQNO (30, 30 pmol l⁻¹). No LLOQ was defined for sputum AQs, The LLOQ for P. aeruginosa measured by qPCR was 100 cells g⁻¹ of sputum. Values under LLOQ were designated a 0. All values had the addition of 1 prior to log transformation.