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. 2021 Oct 25;3(10):000269. doi: 10.1099/acmi.0.000269

Fig. 4.

Fig. 4.

Effect of apo-BLf on the proteases from the CS and OMVs of M. haemolytica A2. Proteolytic activities of the CS and OMVs in zymograms copolymerized with gelatin (a, b) or apo-BLf (c, d), respectively. (a, c). Lane 1, CS proteins from bacteria not incubated with apo-BLf; lanes 2 and 3, CS proteins from bacteria incubated with 1 or 3.5 µM apo-BLf, respectively. (b, d). Lane 1, OMV proteins from bacteria not incubated with apo-BLf; lanes 2 and 3, OMV proteins from bacteria incubated with 1 or 3.5 µM apo-BLf, respectively. Inhibited proteases are marked with an *. A result representative of five independent experiments is shown.