Fig. 5.
Accumulation of wt PLPV in an AGO2-inactivated N. benthamiana line. The wt PLPV was agroinoculated in wt N. benthamiana and in N. benthamiana AGO2-Cr line (with AGO2 inactivated through a CRISPR/Cas9 approach). Northern blot analysis of local (a) and systemic (b) leaves collected at 7 days p.i. and 42 days p.i., respectively, was performed with a PLPV-specific riboprobe. Positions of the PLPV genomic (g) and subgenomic (sg) RNAs are indicated at the right of the blots and ethidium bromide staining of rRNAs is included below the blots as loading controls. Two distinct samples are shown for each virus–plant line combination. Plant batches for each experiment included four–six plants and experiments were repeated at least three times.