Fig 1. ogt-1; eel-1 double mutants have heightened sensitivity to electroshock.

(A) Representative close-up images of the experimental tube and electroshock assay readout of wild-type (N2) C. elegans. The tube contains a liquid M9 solution with copper wire on either side of the tube. There are 3–6 worms for each trial of electroshock administration. Shown in the experimental tube are freeze-frame images of a single N2 worm. The images are still frames in 10 second increments from a video of raw data seen in S1 video. These images are taken before the shock in which the animal moves in a sinusoidal wave pattern (denoted in -10 seconds), during the shock where the worm is exhibiting unilateral body bends or paralysis that characterizes the convulsion phase (denoted at 0 seconds), and after the shock where the animal fully recovers locomotion within ~30 seconds as denoted by the asterisk. Sinusoidal movement rate was calculated based on 1 full sinusoidal movement/10 seconds and standardized to 1 minute. From left to right, image 1 shows the starting point of movement, image 2 is when the worm completed 50% of the movement, and image 3 shows a worm that has reached 1 full sinusoidal movement. (B) Shown are each genotype ran through the electroconvulsive seizure assay for 3 seconds at 47V in M9 solution. Average time of recovery was significantly increased in ogt-1; eel-1 double mutants (n = 33) when compared to wildtype (n = 33), eel-1 null mutants (n = 36), and ogt-1 null mutants (n = 39). Error bars represent standard error of the mean. Significance was determined using a One-Way ANOVA with a Bonferroni test–pairwise. Significance between columns were denoted using a *p<0.05, **p<0.01, ***p<0.001. Data was collected from 6/14/19-6/20/19, separate from Fig 2A.