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. 2021 Nov 15;15:4687–4699. doi: 10.2147/DDDT.S329507

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© 2021 Lu et al.

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The expression of GPR18 was decreased in the bladders and DRGs of rats with CYP-induced cystitis 4, 24, and 48 h after intraperitoneal injection of saline or CYP. (A) Analysis of the mRNA expression of GPR18 in the bladders (n=6). (B and C) Analysis of the protein expression of GPR18 and representative Western blotting images of GPR18 in the bladder (n=6). (D) Analysis of the coexpression of GPR18 (red) and PGP9.5 (green) in normal bladder sections using immunofluorescence. (E) Analysis of the mRNA expression of GPR18 in the L6-S1 DRGs (n=6). (F and G) Analysis of the expression and distribution of GPR18 in the L6-S1 DRGs using immunofluorescence (n=6). The arrows indicate the afferent nerves that supply the bladder. Student's t-test was used for statistical analysis. *P<0.05; **P<0.01; ***P<0.001; ns, no significance.