Figure 1.

Characteristics of WT and mutant recPrP.A, schematics of WT-recPrP, Met4-1-recPrP, and ΔN6-recPrP. B, representative images of one round of PMCA to generate recPrP^Sc. PrP was detected by Western blotting with the 3F10 anti-PrP antibody. The signature PK-resistant band of recPrP^Sc was pointed by an arrow. C, densitometric analysis of PK-resistant bands in B (n = 6). The relative intensity of recPrP^Sc of WT sPMCA products (PK-resistant recPrP/recPrP without PK digestion) was set as 100%. Statistical significance was determined by one-way ANOVA followed by Tukey's multiple comparison test. ∗ represents p < 0.05; ∗∗∗ represents p < 0.01. Error bars indicate standard deviations. Single point in C was from a single PMCA reaction. PK, proteinase K; PMCA, protein misfolding cyclic amplification; recPrP, recombinant PrP.