Figure 6.

Pathogenicity of WT, Met4-1, and ΔN6 recPrP^Scin WT mice.A, the PK-resistant PrP in the inoculum was detected by Western blotting with the 3F10 anti-PrP antibody. B, body weight changes in mice inoculated with indicated inocula. Control mice were inoculated with BSA in PBS (n = 4 mice). C, survival curve (left panel) and average incubation time, survival time, and the clinical duration as indicated (right panel) (n = 12 mice for each group). D, PK-resistant PrP^Sc in the brain cell lysates of mice inoculated with indicated inocula. PrP was detected by Western blotting with the 3F10 anti-PrP antibody. Statistical analysis for the survival curve in C was determined by log-rank test. The rest of the statistical analysis was determined by two-way ANOVA followed by Tukey's multiple comparison test. ∗∗∗ represents p < 0.01; ns represents no statistically significant difference. Error bars indicate standard deviations. BSA, bovine serum albumin; PK, proteinase K; recPrP, recombinant PrP.