Figure 3.
Analysis of deuterated (d9)-oleic acid enrichment within the lipidome of M1 and M2 macrophages reveals marked rewiring of exogenous fatty acid metabolism. M1 and M2 BMDMs were treated with d9-oleic acid for the indicated durations and enrichment within individual TG (A–C), DG (D and E), CE (F), acylcarnitine (G), vinyl-ether glycerophospholipid (H–N), and glycerophospholipid (O and P) was determined by MS lipidomics. Two-way ANOVA with Tukey’s HSD test was used to determine statistically significant differences. ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate significant differences between pairwise comparisons of the indicated groups at the following significance level: p < 0.05, p < 0.01, p < 0.001, and p < 0.00001, respectively. Data are shown as mean ± S.D. (error bars) as well as with individual data points from each biological replicate.