Figure 4.
Analysis of d3-palmitic acid enrichment within the lipidome of M1 and M2 macrophages reveals marked rewiring of exogenous fatty acid metabolism. M1 and M2 BMDMs were treated with d3-palmitic acid for 4 h and enrichment within individual TG (A and B), CE (C), DG (D and E), glycerophospholipid (F and L), ether glycerophospholipid (G), and vinyl-ether glycerophospholipid (H–K) was determined by MS lipidomics. Data was analyzed by unpaired t test with exact p values indicated. Data are shown as mean ± S.D. (error bars) as well as with individual data points from each biological replicate.