| Bacterial and virus strains |
|
Escherichia coli DH10Bac competent cells |
Thermo |
Cat#10361012 |
|
|
|
| Chemicals, peptides, and recombinant proteins |
| Cellfectin II |
Thermo |
Cat#10362100 |
| Polyethylenimidine (PEI MAX) |
Polysciences |
Cat#24765 |
| ESF 921 medium |
Expression Systems |
Cat#96–001 |
| n-Dodecyl-β-D-maltopyranoside (DDM) |
GoldBio |
Cat#DDM |
| Cholesteryl hemisuccinate |
Anatrace |
Cat#CH210 |
|
|
|
| Critical commercial assays |
| ADP-Glo™ Kinase Assay |
Promega |
Cat#V9101 |
|
|
|
| Deposited data |
|
|
| Cryo-EM structure of ATP13A2 in the E2-Pi state (map 1) |
This paper |
EMD-24212 |
| Cryo-EM structure of ATP13A2 in the E2-Pi state (map 2) |
This paper |
EMD-24213 |
| Cryo-EM structure of ATP13A2 in the E2·BeF3− state (map 3) |
This paper |
EMD-24214, PDB: 7N70 |
| Cryo-EM structure of ATP13A2 in the E2-Pi state (map 4) |
This paper |
EMD-24215 |
| Cryo-EM structure of ATP13A2 in the E2-AlF4− state (map 5) |
This paper |
EMD-24216 |
| Cryo-EM structure of ATP13A2 in the E2-AlF4− state (map 6) |
This paper |
EMD-24217, PDB: 7N72 |
| Cryo-EM structure of ATP13A2 in the E1P-ADP-like state (map 7) |
This paper |
EMD-24218, PDB: 7N73 |
| Cryo-EM structure of ATP13A2 D508N mutant in the E1-ATP state (map 8) |
This paper |
EMD-24219, PDB: 7N74 |
| Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 1 (map 9) |
This paper |
EMD-24220, PDB: 7N75 |
| Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1-apo state, Conformation 2 (map 10) |
This paper |
EMD-24221, PDB: 7N76 |
| Cryo-EM structure of ATP13A2 D458N/D962N mutant in the E1·AlF4− state (map 11) |
This paper |
EMD-24222, PDB: 7N77 |
| Cryo-EM structure of ATP13A2 in the E2-Pi state (map 12) |
This paper |
EMD-24223, PDB: 7N78 |
| SDS-PAGE gel image of size-exclusion chromatography fractions (Fig. S1A) |
This paper |
DOI:10.17632/g64mnvkgyk.1 |
|
|
|
| Experimental models: cell lines |
|
|
|
Spodoptera frugiperda (Sf9) |
Expression Systems |
94–001F |
|
|
|
| Software and algorithms |
|
|
| Warp |
Tegunov et al., 2019
|
http://www.warpem.com/warp/
|
| cryoSPARC v2 |
Punjani et al., 2017
|
https://cryosparc.com/
|
| Coot |
Emsley et al., 2010
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| Phenix |
Afonine et al., 2018
|
https://www.phenix-online.org/
|
| MolProbity |
Chen et al., 2010
|
http://molprobity.biochem.duke.edu/
|
| Adaptive Poisson-Boltzmann Solver |
Baker et al., 2001; Dolinksy et al., 2004
|
https://www.poissonboltzmann.org/
|
| PyMOL |
Schrödinger |
https://pymol.org/2/
|
| Caver 3.0 |
Chovancova et al., 2012
|
http://www.caver.cz/
|
| UCSF Chimera |
Pettersen et al., 2004
|
https://www.cgl.ucsf.edu/chimera/
|
| UCSF ChimeraX |
Goddard et al., 2018
|
https://www.cgl.ucsf.edu/chimerax/
|
| R software and drc2 package |
Ritz et al., 2015
|
https://www.r-project.org/
|
| Martini 2.2 |
de Jong et al., 2013
|
http://cgmartini.nl/
|
| Gromacs 2020/3 |
Abraham et al., 2015
|
https://www.gromacs.org/
|
| numpy |
|
https://www.numpy.org
|
| MDAnalysis |
Gowers et al., 2016
|
https://www.mdanalysis.org/
|
| matplotlib |
|
https://www.matplotlib.org
|
|
|
|
| Other |
|
|
| Quantifoil holey carbon grid (R1.2/1.3, Au, 400 mesh) |
Electron Microscopy Sciences |
Cat#Q4100AR1.3 |
| Superose 6 Increase, 10/300 GL |
GE Life Sciences (Cytiva) |
Cat#29–0915–96 |
|
|
|
