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Effects of PRODH ligands on the viability of PC3M cells: (A) compound 1; (B)compound 4, (C) compound 2, (D) L-proline. The compounds were added to PC3M cells at 0, 0.195, 0.781, 3.125, 12.5 or 50 mM and exposed to two 45 min treatments (light bars) or kept in the dark (dark bars) per day for 3 days. The number of viable cells was determined using the MTT assay by absorbance at 560 nm and the data were normalized to a control sample with no added compound.
