Fig. 2.

Increased or decreased expression of Nup88 in HeLa cells affects cell invasion. a HeLa cells overexpressing either GFP or Nup88-GFP were incubated on an extracellular matrix-coated chamber for 72 h. Invasive cells that migrated through the matrix were subjected to Diff-Quik staining (scale bar 50 nm). b The number of invasive cells per unit area was counted. The average number of invasive cells per unit area for cells overexpressing GFP and Nup88-GFP was 366 and 471, respectively. Error bars indicate mean ± SD (n = 3); *p < 0.05. c HeLa cells transfected with control (siCONT) or NUP88-targeted (siNUP88) siRNA were incubated on an extracellular matrix-coated chamber for 72 h. Invasive cells that migrated through the matrix were subjected to Diff-Quik staining (scale bar 50 nm). d The number of invasive cells per unit area was counted. The average number of invasive cells per unit area for siCONT- and siNUP88-transfected cells was 214 and 102, respectively. Error bars indicate mean ± SD (n = 3); **p < 0.01