Fig. 3.

Expression and localization of an NF-κB subunit in HeLa cells overexpressing Nup88. a Expression of the p65 subunit of NF-κB in cell lysates prepared from HeLa cells overexpressing GFP or Nup88-GFP was analyzed by immunoblotting. Unfilled and darker shaded arrowheads indicate GFP and Nup88-GFP, respectively. α-Tubulin was detected as a loading control. b Localization of the p65 subunit in HeLa cells overexpressing GFP or Nup88-GFP. The p65 subunit was detected in the cytoplasmic fraction (Cyt) and nuclear extract (NE) by immunoblotting. Unfilled and darker shaded arrowheads indicate GFP and Nup88-GFP, respectively. Lamin A/C is an indicator that nuclear/cytoplasmic fractionation was successfully performed