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. 2021 Jul 31;156(5):409–421. doi: 10.1007/s00418-021-02020-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Overexpression of Nup88 does not stimulate EMT and vice versa. a Expression of EMT-associated proteins in both HeLa cells (lanes 1 and 2) and MCF-7 cells (lanes 3 and 4) overexpressing GFP (lanes 1 and 3) or Nup88-GFP (lanes 2 and 4) was assessed by immunoblotting. Filled and unfilled arrowheads indicate Nup88-GFP and GFP, respectively. b Expression of endogenous Nup88 in both EMT-uninduced (DDK, lane 1) and EMT-induced (Twist-DDK, lane 2) MCF-7 cells was assessed by immunoblotting. α-Tubulin was detected as a loading control