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Gene and protein expression of MMP-12 in HeLa cells overexpressing Nup88. a Gene expression of MMP-12 in HeLa cells overexpressing either GFP or Nup88-GFP was analyzed by RT real-time PCR. b MMP-12 secreted into the culture medium from HeLa cells overexpressing either GFP or Nup88-GFP was analyzed by immunoblotting. Filled and unfilled arrowheads indicate Nup88-GFP and GFP, respectively. α-Tubulin was detected as a loading control
