Fig. 3.

a MC3T3-E1 cells were treated with 0 or 10⁻⁴ M Dex for 24 h, and the calcium ion influx was detected by Fluo8-AM staining. b MC3T3-E1 cells were treated with 0 or 10⁻⁴ M Dex for 24 h, and the expressions of Orai1 and STIM1 were detected by Western blot. MC3T3-E1 cells were treated with DMSO or 2-APB, and then treated with 0 or 10⁻⁴ M Dex for 24 h. c Western blot of ATF6, phosphorylated PERK (p-PERK), PERK, phosphorylated IRE1 (p-IRE1), and IRE1 in MC3T3-E1 cells. d Western blot of CHOP in MC3T3-E1 cells. e Real-time PCR of CHOP mRNA in MC3T3-E1 cells