Fig. 4.

SSd repressed HSCs autophagy activation by regulating GPER1. A qPCR analysis of ER-α, ER-β and GPER1 mRNA expression level in primary HSCs from hepatic tissues in control mice, CCl₄ mice and SSd-treated mice. B qPCR analysis of GPER1 mRNA expression level in LX-2 cells treated with TGF-β in the absence or presence of SSd. C, D Western blot analysis for GPER1 protein expression level in LX-2 cells treated with TGF-β in the absence or presence of SSd. E, F LC3 expression was detected in LX-2 cells treated with G1 (GPER1 agonist) or G15 (GPER1 antagonist) by IF assay. G Western blot analysis for p62 protein expression level in LX-2 cells treated with G1 or G15. **p < 0.01