Fig. 7.

Dendritic cell subsets in ADA2 deficiency. A Gating strategy for DC subsets. A broad gate containing lymphocyte and monocyte populations was first gated against SSC-A vs FSC-A, followed by single cells gated as FSC-H vs FSC-A. DCs were defined as negative for lineage markers (CD3, CD19, CD20, CD56, CD14, CD235a), and HLA-DR⁺. DCs were gated as CD123 and CD11c to distinguish CD123⁺CD11c⁻ plasmacytoid DCs (pDC) and CD123⁻CD11c⁺ myeloid (conventional) DCs. mDCs were gated as CD1c and CD16 to distinguish CD1c^hi, CD1c^lo/− and CD16⁺CD1c^lo/− mDCs and for CD1c and CD141 to identify CD141⁺ mDCs. B–D Representative flow cytometry plots and summary graphs showing proportions of B Lin⁻HLA-DR⁺ DCs, C pDCs and mDCs within the DC population and D CD1c^hi, CD1c^lo/−, CD16 + CD1c^lo/− and CD141⁺ mDC subsets. Graphs represent mean ± S.E.M.; each symbol represents an individual. Significant differences were determined by Mann–Whitney (B) or multiple (C, D) t-tests (**P < 0.01)