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. 2021 Nov 19;11:22578. doi: 10.1038/s41598-021-01842-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Plasmodium falciparum circumsporozoite (CS) primer set schematics indicating expected PCR amplification products. (B) Agarose gel (1.5%) electrophoresis of amplified products using CS primer set. Lanes 1–6 and 10–16 are a representative subset of the 176 examined P. falciparum sporozoite infected treatment Anopheles gambiae s.s. mosquitoes with a P. falciparum (+) positive and (−) negative control (blank). Lane Ld, 100 bp DNA size marker ladder.