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. 2021 Nov 19;5:97. doi: 10.1038/s41698-021-00236-6

Fig. 2. The expression of immune regulatory proteins (IRPs) is heterogeneous in uterine mesenchymal tumors.

Fig. 2

a Representative IF staining of an IRPs-expressing tumor (left), its corresponding H&E staining (right), and representative images of cells expressing PD-1, B7-H4, and IDO1 (bottom). Upper scale bars indicate 100 μm and lower panel scale bar indicates 20 μm. b–e Quantification of IRP-expressing cells shows a heterogeneous expression of PD-1+ (b), IDO1+ (c), B7-H4+ (d), whereas PD-L1 is not detected in any tumor group (e). f Percentage types of PD-1 expressing cells. g PD-1 expression in FOXP3+ cells. h Representative examples of IHC staining for CD4+ T cells representing high and low infiltration tumors. Scale bar indicates 100 μm. i Quantification of CD4+ T cell density shows heterogeneity in CD4+ expression in all tumor types. j Scatter plot shows the significant correlation between IHC stained CD4+ and IF stained CD8-FOXP3+ and CD8-FOXP3-PD-1+ T cells (r indicates Pearson correlation coefficient). Plots indicate average ± s.d. Significance is indicated as **(P < 0.01).