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. 2021 Nov 19;7:144. doi: 10.1038/s41523-021-00352-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Flowchart of the patient sample availability and methods used in the translational sub-study of the SBG-2004-1 early breast cancer trial; b Overview and workflow of the multiplex fluorescent IHC approach in tissue microarrays; c Representative image of the spatial immune cell distribution and phenotyping according to the (co)expression of the relevant markers; d, e Heatmaps depicting mean cell densities of immune cell subpopulations and expression patterns per tissue compartment (n = 79).