Figure 8.

Cell cycle and apoptosis by flow cytometry after cell exposure to their 24 h lethal concentrations of Mumio. Mumio did not affect the cell cycle of SV-HUC1 cells. Increased G0/G1 and decreased S phase cell populations were observed after exposure of T24 cells to LC₅₀ and LC₉₀. Increased cell numbers in G0/G1 phase and S phase were noted in 5637 cells. Apoptosis analysis revealed significantly fewer viable cells with increased late apoptotic cells after exposure to LC₉₀ of Mumio in SV-HUC1. Significantly fewer viable cells with increased apoptotic cells were observed in T24 cells after incubation with LC₅₀ and LC₉₀ of Mumio. SV-HUC-1—normal urothelial cell line, T24—transitional urinary bladder carcinoma cell line, 5637—grade II urinary bladder carcinoma cell line, LC-lethal concentration,*p < 0.05; **p < 0.01; ***p < 0.001 (compared to control).