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. 2021 Nov 19;11:22624. doi: 10.1038/s41598-021-02050-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Clinical serum 5-HT levels determined by serotonin ELISA assay (Eagle Biosciences, Amherst, NH) in CD patients with or without MAP infection vs healthy control Subjects (N = 40 per each group). Values were pre-tested for normal distribution using the Kolmogorov–Smirnov normality test. Significance among experiments was assessed by two-way analysis of variance (ANOVA) followed by Bonferroni correction test. *P value < 0.05 compared to healthy controls. ^#P value < 0.05 compared to healthy controls or CD MAP negative patients.