Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 19;12:6768. doi: 10.1038/s41467-021-26881-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Schematization of the scRNA-seq profiling strategy. B Left panel: UMAP plot labeled with the identified cell identities and RNA velocity vectors: (i) FOXA2⁺/ARX Floor Plate, (ii) NKX2-1⁺/RAX⁺/SIX6⁺ Ventral tuberal hypothalamic progenitors, (iii) NKX2-1⁺/FOXG1⁺ Ventral telencephalic progenitors, (iv) NKX2-1⁺/NHLH2⁺/OTP⁺ Ventral hypothalamic neurons, (v) TFAP2A⁺/KRT19⁺ Superficial ectoderm, (vi) SOX10⁺/PLP1⁺ Neural Crest, (vii) PAX6⁺/EMX2⁺/OTX2⁺ Dorsal forebrain progenitors, (viii) IRX3⁺/OLIG3⁺ Dorsal thalamic progenitors, (ix) TBR1⁺/LHX1⁺ Dorsal Neurons_1, (x) HES6⁺/DLL3⁺ Dorsal Neurons_2, dorsal and ventral proliferating progenitors, (xi) Dorsal, (xii) Dorsal thalamic, (xiii) Ventral and (xiv) an UnIdentified population (UnId). Right panel: z-score scaled heatmap of marker genes used for cell identities classification. C UMAP plot displaying SHH responsive and unresponsive status, and GLI3 and PTCH1 expression level. SHH responsive and unresponsive status have been computed imposing a threshold based on the normalized distribution of GLI3, GAS1 and PTCH1 counts, where GLI3-GAS1^low and PTCH1^high represent SHH responsive while GLI3-GAS1^high, PTCH1^low unresponsive. D qRT-PCR analysis of SHH responsive genes (GLI1 and GLI3) for NG-T2A-SHH and NG-CNTRL at day 14 of differentiation. The histogram displays the average and SD of differentiated cells exposed to light stimulation or dark control (n = 2 biologically independent samples). .